1. Title : Cell harvest & Protein sampling
2. Objective : 크기에 따라 단백질을 분리하는 SDS-PAGE를 하기위해서 poliovirus에 감염된 BGM cell을 용해시킨 후, 단백질을 추출하고 정량한다.
3. Method
1) Cell harvest
① 배지를 e-tube에 수거한다.
② PBS로 washing 하고 1ml PBS를 추가한다.
③ Scraper로 긁어 모아 e-tube에 담는
harvest and dry harvest is possible with some method. That is, we can fix the ratio with 70% of wet harvest regardless of the length of term (either in short-term or in long-term). Although wet harvest is more beneficial to farmers so every farmer will prefer water harvest to dry harvest, we can avoid the increase of ratio by giving some incentives to dry harvest. In this assumption, answer for Q
To understand cost account that we learned in the class and to know how it used in construction site.
The site has inclination . So, the area which resist high pressure use CIP and the area which resist low pressure use H Pile+Timber.
Using the Top-down method, it can excavate both
side at the same time. They don’t need to excavate
next site. Therefore, it could reduce the d
01 Introduction
- What is Sheet pile?
Board shape’s post used in civil and architecture construction
When digging the foundation and tunnel, prevent to collapse around the ground or ceiling
Use in underwater construction for drainage
(중략)
4. Alternative Method
- Overcoming the downsides
Eliminating Compression Anchored method
Using Released head and Speed type Anchor hea
[5] Consideration – 공사 중 고려사항
5.1. 합벽 시공에 대한 계획
건축외벽과 부지 경계선과의 간격이 좁아서 외벽의 거푸집 작업이나 외벽 시공 후 되메우기 작업이 곤란한 경우 부득이 토류벽과 건축외벽을 합벽으로 시공하게 된다. 이때 흙막이(H-Pile)의 시공오차로 인하여 건축외벽의 단면을 침범